Split green fluorescent protein as a modular binding partner for protein crystallization
نویسندگان
چکیده
منابع مشابه
Split green fluorescent protein as a modular binding partner for protein crystallization
A modular strategy for protein crystallization using split green fluorescent protein (GFP) as a crystallization partner is demonstrated. Insertion of a hairpin containing GFP β-strands 10 and 11 into a surface loop of a target protein provides two chain crossings between the target and the reconstituted GFP compared with the single connection afforded by terminal GFP fusions. This strategy was ...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section D Biological Crystallography
سال: 2013
ISSN: 0907-4449
DOI: 10.1107/s0907444913024608